Journal: Environmental Health Perspectives

Article Title: Effects of Crude Oil/Dispersant Mixture and Dispersant Components on PPAR γ Activity in Vitro and in Vivo : Identification of Dioctyl Sodium Sulfosuccinate (DOSS; CAS #577-11-7) as a Probable Obesogen

doi: 10.1289/ehp.1409672

Figure Lengend Snippet: Adipogenesis following DOSS exposure in vitro . 3T3-L1 cells were grown to confluence over 72 hr in PGM (preadipocyte growth media). RFU, relative fluorescence units (firefly relative to renilla). Cells were either maintained in PGM or treated with MIM (minimal induction media) alone or supplemented with 10, 20, 25, or 50 ppm DOSS or 1 μM Rosi for an additional 3 days before analysis. ( A ) AdipoRed quantification of lipid accumulation as described in “Materials and Methods.” Data are expressed as mean ± SD; n = 5 per group (* p < 0.05 vs. MIM control). ( B ) Representative images of lipid accumulation of cells treated as in A (scale bar = 50 μm).

Article Snippet: For triacylglycerol staining assays to quantify adipogenic differentiation, 3T3-L1 preadipocyte cells (Zenbio) were plated in 48-well plates at a density of 10,000 cells per well in preadipocyte growth medium (PGM; DMEM/F12 supplemented as described above) and grown until confluence.

Techniques: In Vitro, Fluorescence